|
|
|
| Registros recuperados: 992 | |
|
|
| González,Luis F.; Henríquez-Belmar,Francisca; Delgado-Acevedo,Claudia; Cisternas-Olmedo,Marisol; Arriagada,Gloria; Sotomayor-Zárate,Ramón; Murphy,Dennis L.; Moya,Pablo R.. |
| Abstract Background Obsessive–compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1–3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus—brain... |
| Tipo: Journal article |
Palavras-chave: EAAT3 SLC1A1 Neuronal glutamate transporter Obsessive– Compulsive disorder. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100225 |
| |
|
|
| Kang,Youra; Timilshina,Maheshwor; Nam,Tae-gyu; Jeong,Byeong-Seon; Chang,Jae-Hoon. |
| Abstract Background CD4+ T cells play an important role in the initiation of an immune response by providing help to other cells. Among the helper T subsets, interferon-γ (IFN-γ)-secreting T helper 1 (Th1) and IL-17-secreting T helper 17 (Th17) cells are indispensable for clearance of intracellular as well as extracellular pathogens. However, Th1 and Th17 cells are also associated with pathogenesis and contribute to the progression of multiple inflammatory conditions and autoimmune diseases. Results In the current study, we found that BJ-1108, a 6-aminopyridin-3-ol analogue, significantly inhibited Th1 and Th17 differentiation in vitro in a concentration-dependent manner, with no effect on proliferation or apoptosis of activated T... |
| Tipo: Journal article |
Palavras-chave: BJ-1108; Th1/Th17 cell; Differentiation; EAE. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100202 |
| |
|
|
| Xu,Yu‑Dong; Wang,Yu; Yin,Lei‑Miao; Peng,Ling‑Ling; Park,Gyoung‑Hee; Yang,Yong‑Qing. |
| Abstract Background Airway remodeling is a key feature of asthma, characterized by increased proliferation of airway smooth muscle cells (ASMCs). S100A8 is a calcium‑binding protein with a potential to regulate cell proliferation. Here, the effect of exogenous S100A8 protein on the proliferation of ASMCs induced by platelet‑derived growth factor (PDGF) and the underlying molecular mechanism was investigated. Methods Rat ASMCs were cultured with or without a neutralizing antibody to the receptor for advanced glycation end‑products (RAGE), a potential receptor for S100A8 protein. Purified recombinant rat S100A8 protein was then added into the cultured cells, and the proliferation of ASMCs induced by PDGF was detected by... |
| Tipo: Journal article |
Palavras-chave: S100A8 Airway smooth muscle cells Cell proliferation The receptor for advanced glycation end‑ Products Platelet‑ Derived growth factor. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100216 |
| |
|
|
| Wu,Xiaofen; Ruan,Lei; Yang,Yi; Mei,Qi. |
| Abstract Background This study aimed to investigate the gene expression changes associated with carcinoma-associated fibroblasts (CAFs) involving in non-small cell lung carcinoma (NSCLC). Methods We downloaded the GEO series GSE22862, which contained matched gene expression values for 15 CAF and normal fibroblasts samples, and series GSE27289 containing SNP genotyping for four matched NSCLC samples. The differentially expressed genes in CAF samples were identified using the limma package in R. Then we performed gene ontology (GO) and pathway enrichment analysis and protein–protein interaction (PPI) network construction using the identified DEGs. Moreover, aberrant cell fraction, ploidy, allele-specific copy number, and loss of heterozygosity... |
| Tipo: Journal article |
Palavras-chave: Carcinoma-associated fibroblast; Non-small cell lung carcinoma; Differentially expressed genes; Allele-specific copy number; Functional analysis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100204 |
| |
|
|
| Yuan,Feng; Wang,Junfeng; Zhang,Keshi; Li,Zhao; Guan,Zhenpeng. |
| Abstract Background Programmed cell death 5 (PDCD5) is an apoptosis-related gene cloned from TF-1 cells whose primary biological functions are to promote apoptosis and immune regulation. The effects and mechanisms exerted by key mediators of arthritic inflammation remain unclear in PDCD5 transgenic (PDCD5 tg) mice. Results In the current study, PDCD5 tg mice inhibited the progression of adjuvant-induced arthritis, specifically decreasing clinical signs and histological damage, compared with arthritis control mice. Additionally, the ratio of CD4+IFN-γ+ cells (Th1) and CD4+IL-17A+ cells (Th17), as well as the mRNA expression of the pro-inflammatory mediators IFN-γ, IL-6, IL-17A and TNF-α, were decreased in PDCD5 tg mice, while... |
| Tipo: Journal article |
Palavras-chave: Rheumatoid arthritis; Adjuvant-induced arthritis; Programmed cell death 5; T lymphocytes; Inflammatory cytokines. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100229 |
| |
|
|
| Wang,Limei; Wu,Xiuyin; Wang,Ruolin; Yang,Chengzhe; Li,Zhi; Wang,Cunwei; Zhang,Fenghe; Yang,Pishan. |
| Abstract Background: Bromodomain-containing protein 4 (BRD4) inhibition is a new therapeutic strategy for many malignancies. In this study, we aimed to explore the effect of BRD4 inhibition by JQ1 on in vitro cell growth, migration and invasion of salivary adenoid cystic carcinoma (SACC). Methods: The human normal epithelial cells and SACC cells (ACC-LM and ACC-83) were treated with JQ1 at concentrations of 0, 0.1, 0.5 or 1 μM. Cell Counting Kit-8 (CCK-8) assay was performed to evaluate cell proliferation. Cell apoptosis and cell cycle distribution was evaluated by Flow cytometry. Immunofluorescence staining was used to examine the expression of BRD4 in SACC cells. The quantitative real-time polymerase chain reaction (qRT-PCR) assay and western... |
| Tipo: Journal article |
Palavras-chave: Salivary adenoid cystic carcinoma; BRD4 inhibition; Proliferation; Migration; Epithelial-mesenchymal transition. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100213 |
| |
|
|
| Mata-Miranda,Monica Maribel; Vazquez-Zapien,Gustavo Jesus; Rojas-Lopez,Marlon; Sanchez-Monroy,Virginia; Perez-Ishiwara,David Guillermo; Delgado-Macuil,Raul Jacobo. |
| Abstract Background Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells. Results In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed... |
| Tipo: Journal article |
Palavras-chave: Pluripotent stem cells; Differentiated kidney cells; Vibrational spectroscopy; Fourier transform infrared; Principal components analysis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100207 |
| |
|
| |
|
|
| Zhang,Xia; Li,Yuehua; Wang,Dan; Wei,Xiaoer. |
| Abstract Background miR-22 has been shown to be frequently downregulated and act as a tumor suppressor in multiple cancers including breast cancers. However, the role of miR-22 in regulating the radioresistance of breast cancer cells, as well as its underlying mechanism is still not well understood. Methods The expressions of miR-22 and sirt1 at mRNA and protein levels were examined by qRT-PCR and Western Blot. The effects of miR-22 overexpression and sirt1 knockdown on cell viability, apoptosis, radiosensitivity, γ-H2AX foci formation were evaluated by CCK-8 assay, flow cytometry, colony formation assay, and γ-H2AX foci formation assay, respectively. Luciferase reporter assay and qRT-PCR analysis were performed to confirm the... |
| Tipo: Journal article |
Palavras-chave: MiR-22; Tumorigenesis; Radiosensitivity; Breast cancer; Sirt1. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100220 |
| |
|
|
| Li,Pengfei; Yu,Xuejing; Xie,Jianshan; Yao,Xiaolei; Liu,Wenzhong; Yao,Jianbo; Zhu,Zhiwei; Lyu,Lihua. |
| Abstract Background: Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulation of food intake, energy homeostasis, and reproduction. The hens egg-laying rate mainly depends on the developmental status of follicles, expression of CART have not been identified from hen follicles, the regulatory mechanisms of CART biological activities are still unknown. The objective of this study was to characterize the mRNA expression of CART in hen follicular granulosa cells and determine CART peptide localization and regulatory role... |
| Tipo: Journal article |
Palavras-chave: Hen; Follicles; CART; Theca cell; Granulosa cell. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100211 |
| |
|
|
| Kargupta,Roli; Puttaswamy,Sachidevi; Lee,Aiden J.; Butler,Timothy E.; Li,Zhongyu; Chakraborty,Sounak; Sengupta,Shramik. |
| Abstract Background: Multiple techniques exist for detecting Mycobacteria, each having its own advantages and drawbacks. Among them, automated culture-based systems like the BACTEC-MGIT™ are popular because they are inexpensive, reliable and highly accurate. However, they have a relatively long "time-to-detection” (TTD). Hence, a method that retains the reliability and low-cost of the MGIT system, while reducing TTD would be highly desirable. Methods: Living bacterial cells possess a membrane potential, on account of which they store charge when subjected to an AC-field. This charge storage (bulk capacitance) can be estimated using impedance measurements at multiple frequencies. An increase in the number of living cells during culture... |
| Tipo: Journal article |
Palavras-chave: Mycobacteria; Tuberculosis; Electrical impedance spectroscopy; BD Bactec MGIT 960; M. bovis BCG; M. smegmatis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100214 |
| |
|
|
| Quiroz,Karla A.; Berrios,Miguel; Carrasco,Basilio; Retamales,Jorge B.; Caligari,Peter D. S.; García-Gonzáles,Rolando. |
| Abstract Background: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis. Results: In this context, we studied the cultivation of meristems and establishment of a... |
| Tipo: Journal article |
Palavras-chave: Fragaria chiloensis; Meristem culture; Plant growth regulators; Virus elimination; Plant morphogenesis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100404 |
| |
| Registros recuperados: 992 | |
|
|
|
